diff options
Diffstat (limited to 'gnu/packages/bioinformatics.scm')
| -rw-r--r-- | gnu/packages/bioinformatics.scm | 111 |
1 files changed, 93 insertions, 18 deletions
diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm index 28b6cd318b..b913d5647d 100644 --- a/gnu/packages/bioinformatics.scm +++ b/gnu/packages/bioinformatics.scm @@ -7380,13 +7380,13 @@ ID and species. It is used by functions in the GenomeInfoDb package.") (define-public r-genomeinfodb (package (name "r-genomeinfodb") - (version "1.18.0") + (version "1.18.1") (source (origin (method url-fetch) (uri (bioconductor-uri "GenomeInfoDb" version)) (sha256 (base32 - "1xqpgngd8by0yn627v9kz26a03v5a1lhcfwlnx2i0ivplk9bd40s")))) + "049pyzr8iszv3g7wdqf3pz7vg7bzd450c20ln6fgw4g5xnkkr10s")))) (properties `((upstream-name . "GenomeInfoDb"))) (build-system r-build-system) @@ -9748,14 +9748,14 @@ structure (pcaRes) to provide a common interface to the PCA results.") (define-public r-msnbase (package (name "r-msnbase") - (version "2.8.0") + (version "2.8.1") (source (origin (method url-fetch) (uri (bioconductor-uri "MSnbase" version)) (sha256 (base32 - "0nnlydpklmv9kwlk3gkjgabx7l6y4gav3imq98w8wskb1fhm50c0")))) + "0y658anh06vnvbkfs7r8q40gqgyqr2r8kj7jlpnp33fy1lvp1nv7")))) (properties `((upstream-name . "MSnbase"))) (build-system r-build-system) (propagated-inputs @@ -10223,14 +10223,14 @@ originally made available by Holmes, Harris, and Quince, 2012, PLoS ONE 7(2): (define-public r-ensembldb (package (name "r-ensembldb") - (version "2.6.1") + (version "2.6.2") (source (origin (method url-fetch) (uri (bioconductor-uri "ensembldb" version)) (sha256 (base32 - "1js05dcv1dj4g2vj7lzlg0rgjcjlk2irdr9rxqpwxmivm8nbvf36")))) + "0hdz1f34v7sas2v4225icwl3wd4sf17ykpd5dkbx1hc7wcy4w3np")))) (build-system r-build-system) (propagated-inputs `(("r-annotationdbi" ,r-annotationdbi) @@ -11166,7 +11166,7 @@ droplet sequencing. It has been particularly tailored for Drop-seq.") (define-public sambamba (package (name "sambamba") - (version "0.6.7-10-g223fa20") + (version "0.6.8") (source (origin (method git-fetch) @@ -11176,7 +11176,7 @@ droplet sequencing. It has been particularly tailored for Drop-seq.") (file-name (string-append name "-" version "-checkout")) (sha256 (base32 - "1zb9hrxglxqh13ava9wwri30cvf85hjnbn8ccnr8l60a3k5avczn")))) + "0k0cz3qcv98p6cq09zlbgnjsggxcqbcmzxg5zikgcgbr2nfq4lry")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there is no test target @@ -11187,9 +11187,10 @@ droplet sequencing. It has been particularly tailored for Drop-seq.") (add-after 'unpack 'fix-ldc-version (lambda _ (substitute* "gen_ldc_version_info.py" - (("/usr/bin/env.*") (which "python"))) + (("/usr/bin/env.*") (which "python3"))) (substitute* "Makefile" - (("\\$\\(shell which ldmd2\\)") (which "ldmd2"))) + ;; We use ldc2 instead of ldmd2 to compile sambamba. + (("\\$\\(shell which ldmd2\\)") (which "ldc2"))) #t)) (add-after 'unpack 'place-biod-and-undead (lambda* (#:key inputs #:allow-other-keys) @@ -11201,21 +11202,21 @@ droplet sequencing. It has been particularly tailored for Drop-seq.") (substitute* "Makefile" (("htslib/libhts.a lz4/lib/liblz4.a") "-L-lhts -L-llz4") - ((" htslib-static lz4-static") "")) + ((" lz4-static htslib-static") "")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (mkdir-p bin) - (install-file "build/sambamba" bin) + (install-file "bin/sambamba" bin) #t)))))) (native-inputs `(("ldc" ,ldc) ("rdmd" ,rdmd) - ("python" ,python2-minimal) + ("python" ,python-minimal) ("biod" - ,(let ((commit "c778e4f2d8bacea7499283ce39f5577b232732c6")) + ,(let ((commit "4f1a7d2fb7ef3dfe962aa357d672f354ebfbe42e")) (origin (method git-fetch) (uri (git-reference @@ -11226,20 +11227,20 @@ droplet sequencing. It has been particularly tailored for Drop-seq.") "-checkout")) (sha256 (base32 - "1z90562hg47i63gx042wb3ak2vqjg5z7hwgn9bp2pdxfg3nxrw37"))))) + "1k5pdjv1qvi0a3rwd1sfq6zbj37l86i7bf710m4c0y6737lxj426"))))) ("undead" - ,(let ((commit "92803d25c88657e945511f0976a0c79d8da46e89")) + ,(let ((commit "9be93876982b5f14fcca60832563b3cd767dd84d")) (origin (method git-fetch) (uri (git-reference - (url "https://github.com/dlang/undeaD.git") + (url "https://github.com/biod/undeaD.git") (commit commit))) (file-name (string-append "undead-" (string-take commit 9) "-checkout")) (sha256 (base32 - "0vq6n81vzqvgphjw54lz2isc1j8lcxwjdbrhqz1h5gwrvw9w5138"))))))) + "1xfarj0nqlmi5jd1vmcmm7pabzaf9hxyvk6hp0d6jslb5k9r8r3d"))))))) (inputs `(("lz4" ,lz4) ("htslib" ,htslib-for-sambamba))) @@ -14376,3 +14377,77 @@ both read length (longer is better) and read identity (higher is better) when choosing which reads pass the filter.") (license (list license:gpl3 ;filtlong license:asl2.0))))) ;histogram.py + +(define-public nanopolish + ;; The recommended way to install is to clone the git repository + ;; <https://github.com/jts/nanopolish#installing-a-particular-release>. + ;; Also, the differences between release and current version seem to be + ;; significant. + (let ((commit "50e8b5cc62f9b46f5445f5c5e8c5ab7263ea6d9d") + (revision "1")) + (package + (name "nanopolish") + (version (git-version "0.10.2" revision commit)) + (source + (origin + (method git-fetch) + (uri (git-reference + (url "https://github.com/jts/nanopolish.git") + (commit commit) + (recursive? #t))) + (file-name (git-file-name name version)) + (sha256 + (base32 "09j5gz57yr9i34a27vbl72i4g8syv2zzgmsfyjq02yshmnrvkjs6")))) + (build-system gnu-build-system) + (arguments + `(#:make-flags + `("HDF5=noinstall" "EIGEN=noinstall" "HTS=noinstall" "CC=gcc") + #:tests? #f ; no check target + #:phases + (modify-phases %standard-phases + (add-after 'unpack 'find-eigen + (lambda* (#:key inputs #:allow-other-keys) + (setenv "CPATH" + (string-append (assoc-ref inputs "eigen") + "/include/eigen3")) + #t)) + (delete 'configure) + (replace 'install + (lambda* (#:key outputs #:allow-other-keys) + (let* ((out (assoc-ref outputs "out")) + (bin (string-append out "/bin")) + (scripts (string-append out "/share/nanopolish/scripts"))) + + (install-file "nanopolish" bin) + (for-each (lambda (file) (install-file file scripts)) + (find-files "scripts" ".*")) + #t))) + (add-after 'install 'wrap-programs + (lambda* (#:key outputs #:allow-other-keys) + (for-each (lambda (file) + (wrap-program file `("PYTHONPATH" ":" prefix (,path)))) + (find-files "/share/nanopolish/scripts" "\\.py")) + (for-each (lambda (file) + (wrap-program file `("PERL5LIB" ":" prefix (,path)))) + (find-files "/share/nanopolish/scripts" "\\.pl")) + #t))))) + (inputs + `(("eigen" ,eigen) + ("hdf5" ,hdf5) + ("htslib" ,htslib) + ("perl" ,perl) + ("python" ,python) + ("python-biopython" ,python-biopython) + ("python-numpy" ,python-numpy) + ("python-pysam" ,python-pysam) + ("python-scikit-learn" , python-scikit-learn) + ("python-scipy" ,python-scipy) + ("zlib" ,zlib))) + (home-page "https://github.com/jts/nanopolish") + (synopsis "Signal-level analysis of Oxford Nanopore sequencing data") + (description + "This package analyses the Oxford Nanopore sequencing data at signal-level. +Nanopolish can calculate an improved consensus sequence for a draft genome +assembly, detect base modifications, call SNPs (Single nucleotide +polymorphisms) and indels with respect to a reference genome and more.") + (license license:expat)))) |