From 46264c73a6364e0510cbe6841a135da5d7f29dfa Mon Sep 17 00:00:00 2001 From: Ricardo Wurmus Date: Fri, 16 Mar 2018 10:59:17 +0100 Subject: gnu: Add pigx-scrnaseq. * gnu/packages/bioinformatics.scm (pigx-scrnaseq): New variable. --- gnu/packages/bioinformatics.scm | 77 +++++++++++++++++++++++++++++++++++++++++ 1 file changed, 77 insertions(+) (limited to 'gnu/packages/bioinformatics.scm') diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm index 989e963c57..1b49cfdfbf 100644 --- a/gnu/packages/bioinformatics.scm +++ b/gnu/packages/bioinformatics.scm @@ -12857,6 +12857,83 @@ and coverage and can be used to produce information on differential methylation and segmentation.") (license license:gpl3+))) +(define-public pigx-scrnaseq + (package + (name "pigx-scrnaseq") + (version "0.0.2") + (source (origin + (method url-fetch) + (uri (string-append "https://github.com/BIMSBbioinfo/pigx_scrnaseq/" + "releases/download/v" version + "/pigx_scrnaseq-" version ".tar.gz")) + (sha256 + (base32 + "03gwp83823ji59y6nvyz89i4yd3faaqpc3791qia71i91470vfsg")))) + (build-system gnu-build-system) + (arguments + `(#:configure-flags + (list (string-append "PICARDJAR=" (assoc-ref %build-inputs "java-picard") + "/share/java/picard.jar") + (string-append "DROPSEQJAR=" (assoc-ref %build-inputs "dropseq-tools") + "/share/java/dropseq.jar")) + #:phases + (modify-phases %standard-phases + (add-after 'install 'wrap-executable + ;; Make sure the executable finds all R modules. + (lambda* (#:key inputs outputs #:allow-other-keys) + (let ((out (assoc-ref outputs "out"))) + (wrap-program (string-append out "/bin/pigx-scrnaseq") + `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE"))) + `("PYTHONPATH" ":" = (,(getenv "PYTHONPATH"))))) + #t))))) + (inputs + `(("dropseq-tools" ,dropseq-tools) + ("fastqc" ,fastqc) + ("java-picard" ,java-picard) + ("java" ,icedtea-8) + ("python-wrapper" ,python-wrapper) + ("python-pyyaml" ,python-pyyaml) + ("python-pandas" ,python-pandas) + ("python-numpy" ,python-numpy) + ("python-loompy" ,python-loompy) + ("ghc-pandoc" ,ghc-pandoc) + ("ghc-pandoc-citeproc" ,ghc-pandoc-citeproc) + ("snakemake" ,snakemake) + ("star" ,star) + ("r-minimal" ,r-minimal) + ("r-argparser" ,r-argparser) + ("r-cowplot" ,r-cowplot) + ("r-data-table" ,r-data-table) + ("r-delayedarray" ,r-delayedarray) + ("r-delayedmatrixstats" ,r-delayedmatrixstats) + ("r-dplyr" ,r-dplyr) + ("r-dropbead" ,r-dropbead) + ("r-dt" ,r-dt) + ("r-genomicalignments" ,r-genomicalignments) + ("r-genomicfiles" ,r-genomicfiles) + ("r-genomicranges" ,r-genomicranges) + ("r-ggplot2" ,r-ggplot2) + ("r-hdf5array" ,r-hdf5array) + ("r-pheatmap" ,r-pheatmap) + ("r-rmarkdown" ,r-rmarkdown) + ("r-rsamtools" ,r-rsamtools) + ("r-rtracklayer" ,r-rtracklayer) + ("r-rtsne" ,r-rtsne) + ("r-scater" ,r-scater) + ("r-scran" ,r-scran) + ("r-singlecellexperiment" ,r-singlecellexperiment) + ("r-stringr" ,r-stringr) + ("r-yaml" ,r-yaml))) + (home-page "http://bioinformatics.mdc-berlin.de/pigx/") + (synopsis "Analysis pipeline for single-cell RNA sequencing experiments") + (description "PiGX scRNAseq is an analysis pipeline for preprocessing and +quality control for single cell RNA sequencing experiments. The inputs are +read files from the sequencing experiment, and a configuration file which +describes the experiment. It produces processed files for downstream analysis +and interactive quality reports. The pipeline is designed to work with UMI +based methods.") + (license license:gpl3+))) + (define-public r-diversitree (package (name "r-diversitree") -- cgit 1.4.1